The Other Determination Of Chlorophyll Fluorescence Spectra
In recent years, the spectrophotometric determination of seawater chlorophyll a concentration has gradually been more easy and high-sensitivity extraction fluorescence method instead. The principle is to emit red fluorescence of chlorophyll a in acetone extracts by blue excitation fluorescence values before and after the determination of acidification of the extract of the fluorometer at a wavelength of 670nm, calculated seawater chlorophyll a content:
In the type and Rb-acidification before the fluorescence value; Ra-acidification fluorescence; R-pure chlorophyll a acidification factor (varies with the instrument). , So (1) becomes:Where, of Fd-determination fluorometer used a conversion factor of the range (mg/m3), its value There are several ways, which are detailed below:
The standard chlorophyll a calibration method: pure chlorophyll a dubbed the mother liquor, the mother liquor diluted into a series of different concentrations of solution were measured by fluorescence readings, and finally using the linear regression method to calculate the calibration line slope of Soil nutrient analyzer.
spectrophotometer calibration method: take a certain volume of unicellular algae in the exponential growth phase, according to the spectrophotometric determination of chlorophyll a measured absorbance value of E, according to a formula chl-a = 11.85E664-1.54 E647 0.08 E630 = cchl-a calculation of the extract Cchl-a. Then measured in a fluorometer fluorescence values of Rb, obtained by of Fd. In this study, using a spectrophotometer calibration method.